Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.

Xiaoqin Wang, Zhenhua Li, Wenqing Li, Cheng Li, Jing Liu, Yu Lu, Jiangbo Fan, Haiyan Ren, Linjuan Huang, Zhongfu Wang.

Human Milk Proteins: An Interactomics and Updated Functional Overview.

Angelo D’Alessandro, Andrea Scaloni and Lello Zolla.

Proteomic Characterization of Human Milk Fat Globule Membrane Proteins during a 12 Month Lactation Period.

Yalin Liao, Rudy Alvarado, Brett Phinney, and Bo Lönnerdal.

Journal of Agricultural and Food Chemistry 2012, 60 N- and O-Glycosylation of a Commercial Bovine Whey Protein Product. Journal of Agricultural and Food Chemistry 2014, 62 Peptidomic Profile of Milk of Holstein Cows at Peak Lactation. Journal of Agricultural and Food Chemistry 2017, 65 Comparative Glycomics of Fat Globule Membrane Glycoconjugates from Buffalo (Bubalus bubalis) Milk and Colostrum.

Nagaraju Brijesha, Shin-Ichiro Nishimura, and Huligerepura Sosalegowda Aparna.

Decoding of O-Linked Glycosylation by Mass Spectrometry.

SriHariRaju Mulagapati, Veerendra Koppolu, and T.

Comparative Analysis of Whey N-Glycoproteins in Human Colostrum and Mature Milk Using Quantitative Glycoproteomics.

Xueyan Cao, Dahe Song, Mei Yang, Ning Yang, Qing Ye, Dongbing Tao, Biao Liu, Rina Wu, and Xiqing Yue.

Journal of Agricultural and Food Chemistry 2018, 66 Comparative Site-Specific N-Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC–MS/MS Reveals a Distinct Glycan Pattern.

Gnanesh Kumar B S, Prasad Mohan Reddy, Sanjay Kottekad.Journal of Agricultural and Food Chemistry 2019, 67 Mass Spectrometry Analysis of Changes in Human Milk N/O-Glycopatterns at Different Lactation Stages. Yu Lu, Jie Liu, Yue Jia, Yuerong Yang, Qinghui Chen, Lujia Sun, Shuang Song, Linjuan Huang, Zhongfu Wang.Journal of Agricultural and Food Chemistry 2021, 69 Glycoproteomics Analysis Reveals Differential Expression of Site-Specific Glycosylation in Human Milk Whey during Lactation. Szeto, Hongqiang Qin, Yan Jin, Mingliang Ye. Zhongyu Wang, Na Zhang, Wendan Wang, Yitong Li, Ignatius M.This article is cited by 76 publications. The O-linked fucose structure in human milk is the first to our knowledge to be found on high molecular mass mucin-type molecules. In addition, non-mucin-type O-linked fucosylated oligosaccharides were found (NeuAc-Gal-GlcNAc1-3Fuc-ol in bovine milk and Gal-GlcNAc1-3Fuc-ol in human milk).

This data indicates that human milk may provide different innate immune protection against pathogens compared to bovine milk, as evidenced by the presence of Lewis b epitope, a target for the Helicobacter pylori bacteria, on human, but not bovine, milk fat globule membrane mucins. Analysis of bovine colostrum showed that O-linked core 2 oligosaccharides are present at the early stage (3 days after birth) but are down-regulated as lactation develops. The O-linked structures were found to be attached to the high molecular mass mucins isolated by agarose-polyacrylamide composite gel electrophoresis, where MUC1 and MUC4 were present. These epitopes were also presented on the reducing terminus of the human, but not the bovine, N-linked oligosaccharides. The C-6 branch of these structures was extended with branched and unbranched N-acetyllactosamine units terminating in blood group H and Lewis type epitopes. Global release of N-linked and O-linked oligosaccharides showed both to be highly sialylated, with bovine peak-lactating milk O-linked oligosaccharides presenting as mono- and disialylated core 1 oligosaccharides (Galβ1-3GalNAcol), while human milk had core type 2 oligosaccharides (Galβ1-3(GlcNAcβ1-6)GalNAcol) with sialylation on the C-3 branch. Oligosaccharides from human and bovine milk fat globule membranes were analyzed by LC-MS and LC-MS/MS.

Cheng yu kessen ii